Combining single‐cell sequencing to identify key immune genes and construct the prognostic evaluation model for colon cancer patients

Colon cancer is a common tumor with high incidence worldwide.1,2 Recently, Immunotherapy has been found to be an effective anti-cancer method, but it has not been used well in the treatment of colon cancer. [...]finding new prognostic-related immune checkpoint genes in colon cancer is particularly important. CD4T and Th1 cells are inextricably linked to the development of colon cancer, which has been proved to promote the precancerous lesions of colon cancer-the pathogenesis of inflammatory bowel disease.4 The inhibition of TIGIT enhances tumor-specific T cellular immunity, so can enhance PD-1 ligand PD-L1 antibody therapy, thus enhancing the sustained memory immune function of tumor reactivation.5 LAG3 high expression indicates better prognostic in colon cancer patients.6 Besides, we found a high positive correlation between TRGC2, CD3E and TIGIT, PDCD1, LAG3. [...]combined with the data of single cell sequencing, our study identified 11 immune-related genes significantly associated with the prognosis of colon cancer patients and constructed the prognostic evaluation model.