OUTBREAK OF cagA AND iceA IN H. PYLORI STRAINS ISOLATED FROM PATIENTS WITH GASTRO DUODENAL DISEASES IN BABOL CITY

BACKGROUND AND OBJECTIVE: Helicobacter pylori is one of the most common bacteria that afflicts world’ human population. Virulence factor of Helicobacter pylori have been associated with clinical outcome of the infection including gastritis, gastric ulcer (GU), duodenal ulcer (DU) and gastric cancer. The aim of the present study was to determine the outbreak of cagA and iceA genotypes of H. pylori in patients with gastroduodenal diseases in Babol, North of Iran.METHODS: Fifty patients with dyspepsia underwent gastroscopy and antral biopsy for histologic study. CagA, iceA, iceA1 and iceA2 genotypes were determined by PCR. FINDINGS: Of 30(60%) HP positive strains, 80% were positive for cagA gene. CagA genotype was positive in 91.7% (11 of 12), 73.3% (11 of 15) and 66.7% (2 of 3) of patients with gastritis, DU and GU, respectively. IceA was positive in 21(70%) of all patients and iceA1 was found in 14(66.7%) isolates and iceA2 was found in 23.8% isolates. IceA1 and IceA2 were not found in 2(9.5%). IceA1 outbreak in patients with gastritis, DU and GU was 88.9%, 45.5% and 50%, respectively.CONCLUSION: Positive cagA strain was more than negative cagA and, iceA1 was predominant strains in our patients with HP infection. We found that two genes other than iceA1 and iceA2 in this study. It seems that new allele other than iceA1 and iceA2 genes are present in H. pylori.