Blood plasma proteins and protein fractions in roe deer Capreolus capreolus L

The aim of the research was to investigate some selected biochemical blood parameters in roe deer (Capreolus capreolus L.). The experiment covered 15 from 2 to 3-year-old bucks from Kuyavian-Pomeranian Voivodeship. The animals were shot by individual hunters on the shooting grounds during the hunting season of 2008/2009 (in the accordance with the Journal of Laws No 48). The material for the research was blood plasma obtained after centrifuging full, nonhemolyzed blood. The blood was collected from the zygomatic vein directly to the test tubes with EDTA and transported in cooling conditions to the laboratory. After transporting the samples of blood to a certified analytical laboratory, the following elements of the obtained blood plasma were examined: ceruloplasmin – using turbidimetric method; transferrin – using immunoturbimetric method; troponin- using a third generation assay on an Elecsys; total protein, albumin, globulin – using spectrophotometric method and total iron – using colorimetric method. The results were statistically analyzed, i.e. the correlation between the parameters was measured by means of Pearson’s correlation coefficient. The analysis of the results revealed a number of statistically significant relations between the parameters under the investigation, especially among the compounds directly responsible for metabolism of iron and copper. A statistically important positive correlation was observed between ceruloplasmin and ferritin (r = 0.563; P≤0.05) and a negative one between transferrin and troponin (r = -0.609; P≤0.05). Moreover, the content of transferrin – an iron-binding protein – was 0.17 g/l, while the concentration of iron was 58 μmol/l. The content of ceruloplasmin – a protein responsible for metabolism of copper – was very low (0.036 g/l). The level of proteins in the blood plasma of the animals under the research was approximately 72 g/l, with the share of albumins about 46%. The albumin-globulin ratio was 0.86.