First description of a mobatvirus (Hantaviridae) in the Amazon region

•This is the first report of a mobatvirus (Hantaviridae) in the Amazon.•The second in Brazil, 881 km from the first Buritiense virus (BURV) detection site.•It is also the first detection of BURV in a Carollia brevicauda bat.•New information was obtained on the M and S segments of its genome. From 1988–2023, Pará accounted for the highest proportion of deforestation among all states in the Brazilian Amazon. Specifically, 57.20 % of the territory in the Santa Bárbara municipality was deforested as of 2023. Since 2017, the Hantaviridae family has included viruses identified in nonrodent vertebrates, such as mobatviruses (from moles and bats). In the current metagenomic analysis of a pool of multiple organs of a Carollia brevicauda bat, we obtained sequences for three segments of the previously described Buritiense virus (BURV); to date, only the L segment had been sequenced for this mobatvirus. This study provides the first description of BURV in the Amazon region and provides information on the S and M segments of the virus. These findings corroborate the presence of BURV in Brazil in an area far from the site of the first detection and in another bat species.