Technology Invention and Mechanism Analysis of Rapid Rooting of Taxus × media Rehder Branches Induced by Agrobacterium rhizogenes

, a vital source of the anticancer drug paclitaxel, grapples with a pronounced supply-demand gap. Current efforts to alleviate the paclitaxel shortage involve expanding cultivation through cutting propagation. However, traditional cutting propagation of is difficult to root and time-consuming. Obtaining the roots with high paclitaxel content will cause tree death and resource destruction, which is not conducive to the development of the industry. To address this, establishing rapid and efficient stem rooting systems emerges as a key solution for propagation, facilitating direct and continuous root utilization. In this study, were induced in the 1-3-year-old branches of × Rehder, which has the highest paclitaxel content. The research delves into the rooting efficiency induced by different strains, with MSU440 and C58 exhibiting superior effects. Transcriptome and metabolome analyses revealed ' impact on hormone signal transduction, amino acid metabolism, zeatin synthesis, and secondary metabolite synthesis pathways in roots. LC-MS-targeted quantitative detection showed no significant difference in paclitaxel and baccatin III content between naturally formed and induced roots. These findings underpin the theoretical framework for rapid propagation, contributing to the sustainable advancement of the industry.