Preliminary researches on conditions to extract alchol soluble proteins of leaves of Wedelia trilobata (L.) Hitchc

The conditions to extract alchol soluble proteins of leaves of Wedelia trilobata (L.) Hitchc. were discussed. When the extractant was alcohol, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10, temperature for extraction was 50°Cand the extraction period was 2hrs, the concentration of alcohol solution needed for the best extraction effect was 95% (V/V). When the extractant was 75% alcohol solution, the temperature for extraction was 50°Cand the extraction period was 2hrs, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio needed for the best extraction effect was 1:20. When the extractant was 75% alcohol solution, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10 and the extraction period was 2 hrs, the temperature needed for the best extraction effect was 55°C. When the extractant was 75% alcohol solution, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10 and the temperature for extraction was 50°C, the extraction period needed for the best extraction effect was 2hrs.