Priority establishment of soil bacteria in rhizosphere limited the spread of tetracycline resistance genes from pig manure to soil-plant systems based on synthetic communities approach

[Display omitted] •Pig manure bacteria were tracked in a soil–plant system based on SynCom approach.•Firmicutes of SynCom were efficiently filtered out in the rhizosphere.•Invasion of SynCom spread TRGs from pig manure to soil and plant.•Priority establishment of soil bacteria limited spread of manu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Environment international 2024-05, Vol.187, p.108732-108732, Article 108732
Hauptverfasser: Wen, Xin, Xu, Jiaojiao, Worrich, Anja, Li, Xianghui, Yuan, Xingyun, Ma, Baohua, Zou, Yongde, Wang, Yan, Liao, Xindi, Wu, Yinbao
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:[Display omitted] •Pig manure bacteria were tracked in a soil–plant system based on SynCom approach.•Firmicutes of SynCom were efficiently filtered out in the rhizosphere.•Invasion of SynCom spread TRGs from pig manure to soil and plant.•Priority establishment of soil bacteria limited spread of manured TRGs in systems.•Doxycycline promoted HGT of the resistance plasmid RP4 within SynCom in A. thaliana. The spread of antibiotic resistance genes (ARGs) in agroecosystems through the application of animal manure is a global threat to human and environmental health. However, the adaptability and colonization ability of animal manure-derived bacteria determine the spread pathways of ARG in agroecosystems, which have rarely been studied. Here, we performed an invasion experiment by creating a synthetic communities (SynCom) with ten isolates from pig manure and followed its assembly during gnotobiotic cultivation of a soil-Arabidopsis thaliana (A. thaliana) system. We found that Firmicutes in the SynCom were efficiently filtered out in the rhizosphere, thereby limiting the entry of tetracycline resistance genes (TRGs) into the plant. However, Proteobacteria and Actinobacteria in the SynCom were able to establish in all compartments of the soil–plant system thereby spreading TRGs from manure to soil and plant. The presence of native soil bacteria prevented the establishment of manure-borne bacteria and effectively reduced the spread of TRGs. Achromobacter mucicolens and Pantoea septica were the main vectors for the entry of tetA into plants. Furthermore, doxycycline stress promoted the horizontal gene transfer (HGT) of the conjugative resistance plasmid RP4 within the SynCom in A. thaliana by upregulating the expression of HGT-related mRNAs. Therefore, this study provides evidence for the dissemination pathways of ARGs in agricultural systems through the invasion of manure-derived bacteria and HGT by conjugative resistance plasmids and demonstrates that the priority establishment of soil bacteria in the rhizosphere limited the spread of TRGs from pig manure to soil-plant systems.
ISSN:0160-4120
1873-6750
DOI:10.1016/j.envint.2024.108732