Easy-Prime: a machine learning-based prime editor design tool

Prime editing is a revolutionary genome-editing technology that can make a wide range of precise edits in DNA. However, designing highly efficient prime editors (PEs) remains challenging. We develop Easy-Prime, a machine learning-based program trained with multiple published data sources. Easy-Prime...

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Veröffentlicht in:Genome Biology 2021-08, Vol.22 (1), p.235-11, Article 235
Hauptverfasser: Li, Yichao, Chen, Jingjing, Tsai, Shengdar Q, Cheng, Yong
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Sprache:eng
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Zusammenfassung:Prime editing is a revolutionary genome-editing technology that can make a wide range of precise edits in DNA. However, designing highly efficient prime editors (PEs) remains challenging. We develop Easy-Prime, a machine learning-based program trained with multiple published data sources. Easy-Prime captures both known and novel features, such as RNA folding structure, and optimizes feature combinations to improve editing efficiency. We provide optimized PE design for installation of 89.5% of 152,351 GWAS variants. Easy-Prime is available both as a command line tool and an interactive PE design server at: http://easy-prime.cc/ .
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-021-02458-0