Evaluation of biobutanol production by Clostridium beijerinckii NRRL B-592 using sweet sorghum as carbon source

In this research it was evaluated the production of biobutanol by Clostridium beijerinckiiNRRL B-592 using sweet sorghum juice as carbon source. Operational variables, like pH and initial inoculum size, as well as supplementation of industrial media with yeast extract and tryptone, were evaluated. T...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Ciência rural 2015-09, Vol.45 (9), p.1707-1712
Hauptverfasser: Visioli, Luiz Jardel, Alves, Eliana Albornoz, Trindade, Aline, Kuhn, Raquel Cristine, Schwaab, Marcio, Mazutti, Marcio Antonio
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In this research it was evaluated the production of biobutanol by Clostridium beijerinckiiNRRL B-592 using sweet sorghum juice as carbon source. Operational variables, like pH and initial inoculum size, as well as supplementation of industrial media with yeast extract and tryptone, were evaluated. The maximum butanol obtained was 2.12g kg-1 using 12.5% of inoculum size, 0.05g 100mL-1 of tryptone and 0.1g 100mL-1 of yeast extract and initial pH of 5.5. The main contribution of this research was to show a systematic procedure for development of a low cost industrial media for biobutanol production from sweet sorghum. Neste trabalho, foi avaliada a produção de biobutanol por Clostridium beijerinckiiNRRL B-592 usando o caldo de sorgo sacarino como fonte de carbono. Foram avaliadas as variáveis operacionais como pH e densidade de inóculo, bem como a suplementação de meios industriais com extrato de levedura e triptona. A máxima concentração de butanol obtida foi de 2,12g kg-1, utilizando 12,5% de inóculo, 0,05g 100mL-1 de triptona e 0,1g 100ml-1 de extrato de levedura e pH inicial de 5,5. A principal contribuição deste trabalho foi o de mostrar um procedimento sistemático para o desenvolvimento de um meio industrial de baixo custo para a produção de biobutanol a partir de sorgo sacarino.
ISSN:1678-4596
1678-4596
DOI:10.1590/0103-8478cr20140520