T1-Mapping and extracellular volume estimates in pediatric subjects with Duchenne muscular dystrophy and healthy controls at 3T

Background Cardiovascular disease is the leading cause of death in patients with Duchenne muscular dystrophy (DMD)--a fatal X-linked genetic disorder. Late gadolinium enhancement (LGE) imaging is the current gold standard for detecting myocardial tissue remodeling, but it is often a late finding. Current research aims to investigate cardiovascular magnetic resonance (CMR) biomarkers, including native (pre-contrast) T.sub.1 and extracellular volume (ECV) to evaluate the early on-set of microstructural remodeling and to grade disease severity. To date, native T.sub.1 measurements in DMD have been reported predominantly at 1.5T. This study uses 3T CMR: (1) to characterize global and regional myocardial pre-contrast T.sub.1 differences between healthy controls and LGE + and LGE- boys with DMD; and (2) to report global and regional myocardial post-contrast T.sub.1 values and myocardial ECV estimates in boys with DMD, and (3) to identify left ventricular (LV) T.sub.1-mapping biomarkers capable of distinguishing between healthy controls and boys with DMD and detecting LGE status in DMD. Methods Boys with DMD (N = 28, 13.2 [+ or -] 3.1 years) and healthy age-matched boys (N = 20, 13.4 [+ or -] 3.1 years) were prospectively enrolled and underwent a 3T CMR exam including standard functional imaging and T.sub.1 mapping using a modified Look-Locker inversion recovery (MOLLI) sequence. Pre-contrast T.sub.1 mapping was performed on all boys, but contrast was administered only to boys with DMD for post-contrast T.sub.1 and ECV mapping. Global and segmental myocardial regions of interest were contoured on mid LV T.sub.1 and ECV maps. ROI measurements were compared for pre-contrast myocardial T.sub.1 between boys with DMD and healthy controls, and for post-contrast myocardial T.sub.1 and ECV between LGE + and LGE- boys with DMD using a Wilcoxon rank-sum test. Results are reported as median and interquartile range (IQR). p-Values < 0.05 were considered significant. Receiver Operating Characteristic analysis was used to evaluate a binomial logistic classifier incorporating T.sub.1 mapping and LV function parameters in the tasks of distinguishing between healthy controls and boys with DMD, and detecting LGE status in DMD. The area under the curve is reported. Results Boys with DMD had significantly increased global native T.sub.1 [1332 (60) ms vs. 1289 (56) ms; p = 0.004] and increased within-slice standard deviation (SD) [100 (57) ms vs. 74 (27) ms; p = 0.001] compared to he