Chemical constituents and biological activities of endophytic fungi from Fagopyrum dibotrys

is an important wild food and feed germplasm resource. It has high nutritional and medicinal value and is rich in natural products, including flavonoids, phenolic acids, coumarins, and alkaloids. Endophytic fungi in have emerged as valuable sources of natural products. However, studies on the biological activity and chemical composition of these endophytic fungi remain limited. In this paper, a new method to obtain natural active ingredients by fermentation of endophytic fungi from medicinal plants was proposed. Then the antioxidant and pathogenic activities of the endophytic fungi extracts were determined . In addition, secondary metabolites produced by endophytic fungi with medicinal activity were analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS). Among the 95 endophytic fungal strains in , four strains with high phenol yields were selected by reaction: (J2), (J15), (J74), and (J61). Compared with those of various extracts, the ethyl acetate fractions of (J2), (J15), and (J61) exhibited superior antioxidant and antibacterial properties. The results indicated that the fungal extract was an excellent natural antioxidant and might be a potential antibacterial agent. The DPPH free radical clearance of was 94.96 ± 0.004%. These findings indicated that had strong antioxidant activity. In addition, the extract of had good antibacterial activity against and , with MICs of 0.5 and 0.05 mg/mL, respectively. The chemical constituents of the ethyl acetate extract from were further analyzed by liquid chromatography-mass spectrometry (LC-MS). We noted that can create a variety of medicinal substances that have high value in medicine, such as caffeic acid (884.75 ng/mL), 3-phenyllactic acid (240.72 ng/mL) and norlichexanthone (74.36 ng/mL). In summary, many valuable active substances and medicinal substances can be obtained through the study of endophytic fungi of .