Salidroside inhibits the proliferation and epithelial mesenchymal transformation of NSCLC cell by negatively regulation of TACC2 expression

Objective To investigate the effect of salidroside (SAL) on the proliferation, apoptosis and epithelial mesenchymal transformation (EMT) of non-small cell lung cancer (NSCLC) cell line and to clarify the molecular mechanism. Methods The cancer tissues from 22 lung cancer patients and adjecent tissues were collected, and the different lung cancer cell lines (A549, H358, H1299, H1650) were cultured in vitro. RT-qPCR was used to detect the expression of transforming acidic coiled-coid protein 2(TACC2) in cancer tissues and the lung cancer cell lines. After cells (A549 and H1299) were treated with SAL at different concentrations, the apoptosis and proliferation rates were analyzed by flow cytometry and CCK-8, respectively. The expression of E-cadherin, N-cadherin, TACC2 and the phosphorylation level of P38/MAPK in NSCLC cell were detected by Western blot. Plasmid (pcDNA3.0-TACC2) was transfected into A549 and H1299 cell to detect the effects of TACC2 over-expression on the levels of apoptosis, proliferation and E