Illuminating plant–microbe interaction: How photoperiod affects rhizosphere and pollutant removal in constructed wetland?

[Display omitted] •Photoperiod effect on pollutant removal in CWs was studies.•15-hour photoperiod showed highest COD, TN, and NO3–-N removal efficiency.•15-hour photoperiod promoted microbial nitrification and denitrification in rhizosphere.•15-hour photoperiod resulted in more stable microbial network with Phaselicystis as key connector.•Plant-microbe interactions were of importance for pollutants removal and design considerations. Rhizosphere is a crucial area in comprehending the interaction between plants and microorganisms in constructed wetlands (CWs). However, influence of photoperiod, a key factor that regulates photosynthesis and rhizosphere microbial activity, remains largely unknown. This study investigated the effect of photoperiod (9, 12, 15 h/day) on pollutant removal and underlying mechanisms. Results showed that 15-hour photoperiod treatment exhibited the highest removal efficiencies for COD (87.26%), TN (63.32%), and NO3–-N (97.79%). This treatment enhanced photosynthetic pigmentation and root activity, which increased transport of oxygen and soluble organic carbon to rhizosphere, thus promoting microbial nitrification and denitrification. Microbial community analysis revealed a more stable co-occurrence network due to increased complexity and aggregation in the 15-hour photoperiod treatment. Phaselicystis was identified as a key connector, which was responsible for transferring necessary carbon sources, ATP, and electron donors that supported and optimized nitrogen metabolism in the CWs. Structural equation model analysis emphasized the importance of plant–microbe interactions in pollutant removal through increased substance, information, and energy exchange. These findings offer valuable insights for CWs design and operation in various latitudes and rural areas for small-scale decentralized systems.