Effects of Lacidophilin on Inflammatory and Intestinal Microflora in Mice with Helicobacter pylori Induced Gastritis

Objective: The aim of this study was to investigate the effect of lacidophilin on inflammation and intestinal microflora in H. pylori-induced gastritis mice. Methods: Established an H. pylori-infected mice model by orally administering an H. pylori suspension. The mice were randomly divided into a model group and a lacidophilin treatment group, which received treatment for four weeks. Gram staining, Warthin-Starry silver staining, and H. pylori immunohistochemical staining were conducted to confirm the colonization of H. pylori in the gastric mucosa of mice. Hematoxylin and eosin (HE) staining was performed to observe any histopathological changes in the gastric mucosa of mice. Immunohistochemistry was carried out to observe the expression of iNOS, IL-1β, and 3-Nitrotyrosine in the gastric mucosa of mice. Additionally, the structure of intestinal microflora was analyzed using 16S rRNA sequencing. Results: The successful establishment of H. pylori-infected mice. Lacidophilin improved the stomach histopathologi