EFECTO DEL EXTRACTO BUTANÓLICO DE PEPEROMIA DOLABRIFORMIS SOBRE EL ADN DE LINFOCITOS HUMANOS EVALUADOS POR EL ENSAYO COMETA

Para evaluar el daño genotóxico del extracto butanólico de Peperomia dolabriformis, se utilizaron linfocitos de sangre periférica humana, los que se cultivaron en el medio "PB-max karyotyping medium" durante 24 horas a 37[grados]C. Se usó buffer fosfato y peróxido de hidrógeno como controles negativo y positivo. Los grupos experimentales fueron T1, T2 y T3 con extracto butanólico a las concentraciones de 0,00125%, 0,0025% y 0,01% respectivamente. Los linfocitos embebidos en agarosa LMP a 37[grados]C fueron lisados y sometidos a electroforesis alcalina a 300 mA, 20 V por 40 minutos. Las láminas se colorearon con naranja de acridina, se observaron en microscopio de fluorescencia y se analizaron con el programa "Comet Score". El mayor daño genotóxico al ADN se presentó en la concentración 0,01% con un 65,06% de "DNA in Tail" y 26,49% en el "Tail Moment". Palabras clave: Extracto butanólico, ensayo cometa, genotoxicidad, linfocitos, Peperomia dolabriformis The purpose of this research was to evaluate the effect of butanolic extract from Peperomia dolabriformis on the DNA from human lymphocytes. The DNA damage comet assay was evaluated in human peripheral blood lymphocytes that were cultured in PB-max karyotyping medium and incubated during 24 hours at 37[grados]C. Phosphate buffer and 100 uM-hydrogen peroxide were the negative and positive controls. Treatment groups contained 0.00125%, 0.0025% and 0.01% butanolic extract, respectively. Lymphocytes embedded in 37[grados]C-LMP agarose were lysed and subjected to electrophoresis under alkaline conditions at 300 mA, 20 V for 40 minutes. Acridine orange stained slides were examined using a fluorescent microscope, and the DNA damage was analyzed by the comet score software. The 0.01%-butanolic extract damaged the DNA with 65.06% in the Tail of the comet and 26.49 in the Tail Moment. Keywords: Butanolic extract, comet assay, genotoxic, lymphocytes, Peperomia dolabriformis