Efecto de diferentes sustratos en la aclimatación y costos de plantas micropropagadas de caña flecha (Gynerium sagitatum Aubl)

To reduce costs associated to ex vitro adaptation of arrow cane (Gynerium sagitatum Aubl.) plants Cv “Criolla”, the effect of three substrate mixes (Peat, peat + river sand and peat + rice husk) on survival, plant height and substrate associated plant cost were evaluated. Plants were micropropagated in semisolid MS medium supplied with 0.5 mg L-1 BAP. After medium removal, plants were transferred on 72-plug plastic trays filled with the respective substrate treatment. Trays were covered with translucent plastic covers during three days. Thereafter, plants were maintained in a 50% light shade house, fog irrigated twice a day for 1 minute each during 8 weeks. Treatments were distributed in with a complete randomized block design. Data were analyzed with ANOVA and means were separated with Tukey ́s mean separation test. Results allowed to evidence that peat + sand resulted in significant increase in survival, plant height and approximately 35% decrease in substrate associated plant cost during acclimatization to ex vitro conditions.Keywords: Arrow cane; Micropropagation; Plantlet;Survival; Transplant ex vitro.Temas Agrarios. Enero - Junio 2020; Vol. 25. No. 1, pp. 77-84 | ISSNe 2389-9182 | https://doi.org/10.21897/rta.v25i1.2210ORIGINAL ARTICLETemas Agrarios 2020. This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/deed.es), which allows copying, redistribution, remixing, transformation and creation from the material, on a non-commercial way, with appropriate credit and licensing to the authors of the work. Con el fin de reducir los costos de adaptación ex vitro de plantas micropropagadas de caña flecha (Gynerium sagitatum Aubl.) cultivar “Criolla”, el efecto de tres sustratos (Turba, turba + arena y turba + cascarilla de arroz) sobre la supervivencia, el crecimiento y el costo de las plantas asociado al sustrato fueron evaluados. Las plantas fueron micropropagadas en medio MS semisólido adicionado con 0.5 mg L-1 de BAP. Después de remover el medio de cultivo, las plantas fueron transferidas en bandejas plásticas de 72 alveolos dispensadas con la mezcla de sustrato respectiva. Las bandejas fueron cubiertas con tapas plásticas transparentes durante 3 días. Las plantas fueron mantenidas en una casa malla con polisombra del 50% de luminosidad y dos riegos diarios de 1 minuto cada uno por nebulización durante 8 semanas. El diseño utilizado fue el de bloques al azar, los datos