Cryopreservation of bovine spermatogenic cells using different protective molecules

The objective of this study was to isolate mechanically bovine spermatogenic cells and assess their viability before and after cryopre-servation with three different cryoprotector molecules. The isolated cells presented an initial viability of 76.5%; after cryopreservation it was 51.7%, 55.5% and 58...

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Veröffentlicht in:Archivos de zootecnia 2011, Vol.60 (229), p.293-296
Hauptverfasser: Barbosa, A. P. M, Sereno, J. R. B, Martins, C. F
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Sprache:eng
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Zusammenfassung:The objective of this study was to isolate mechanically bovine spermatogenic cells and assess their viability before and after cryopre-servation with three different cryoprotector molecules. The isolated cells presented an initial viability of 76.5%; after cryopreservation it was 51.7%, 55.5% and 58.8%, respectively for solutions with DMSO, propanodiol, and DMSO with propanodiol. The solution of DMSO with propanodiol was significantly better (p Objetivou-se isolar mecanicamente as células espermatogênicas bovinas e avaliar sua viabilidade antes e após a criopreservação com três diferentes moléculas crioprotetoras. As células isoladas apresentaram uma viabilidade inicial média de 76,5% e após a criopreservação de 51,7%; 55,5% e 58,8%, respectivamente para as soluções de DMSO, propanodiol e DMSO com propanodiol, onde a solução de DMSO com propanodiol foi significativamente superior (p
ISSN:0004-0592