Detección de marcadores genéticos asociados a la resistencia a patógenos en frijol ayocote de Puebla, México

Ayocote beans (Phaseolus coccineusL.); 2n= 2x= 22) is a legume native to the highlands of Mexico, it is important in human nutrition and detection of improvement characteristics, it shows resistance to pathogens and certain abiotic factors that cause losses in the bean crop. With the premise of identifying Phaseolusgermplasm with multiple resistance to biotic and abiotic adverse factors, this work was carried out with the objective of this work was to determine the resistance or susceptibility to two important pathogens: common blight [Xanthomonas axonopodispv. phaseoli(Smith)] and anthracnose [Colletotrichum lindemutianumSacc. & Magn (anamorph Glomerella lindemuthianaShear)] based on the identification of sequences characterized by amplified regions (SCARs). The study included 117 accessions collected at different sites in Puebla, Mexico (region called Carso Huasteco), which were analyzed with 10 SCAR markers (six markers for common blight: SAP6, BAC6, SU91, LG5, R7313, R4865, four for anthracnose:SAS13, SBB14, SAB3, SH18) at the Center for Genomic Biotechnology in Reynosa, Tamaulipas. The SAS13 and SBB14 anthracnose resistance markers are present more frequently (89 and 74%), followed by the common blight resistance markers BAC6 and SU91 (74 and 42%).The germplasm of Zacapoaxtla and Tlatlauquitepec had a higher frequency of SCARs (Zacapoaxtla: 90% and 100% for SAS13 and SBB14;Tlatlauquitepec: 94% and 56%). Accessions with five SCARs could be used as a source of resistance to diseases in Phaseolusand early and late genotypes to flowering and physiological maturity, as well as variables in testa color, pod size and seed, but no association was detected between the presence of SCARs with the morphology of ayocote beans El frijol ayocote (Phaseolus coccineus L.); 2n= 2x= 22) es una leguminosa de las zonas altas de México, tiene importancia en la alimentación humana y detección de características de mejoramiento, muestra resistencia a patógenos y a ciertos factores abióticos que ocasionan pérdidas en el cultivo de frijol. Con la premisa de identificar el germoplasma de Phaseolus con resistencia a factores adversos bióticos y abióticos, el objetivo fue determinar la resistencia a dos patógenos importantes: tizón común [Xanthomonas axonopodis pv. phaseoli (Smith)] y antracnosis [Colletotrichum lindemutianum Sacc. & Magn (anamorfo G. lindemuthiana Shear)] con base a la identificación de secuencias caracterizadas de regiones amplificadas (SCARs). El estudio inclu