Evaluación in vitro de la microfiltración de Enterococcus faecalis usando cinco técnicas de obturación

Background: The root canal filling technique named Hybrid-Mixed Condensation, combines the advantages of cold lateral and warm vertical condensation. The ability of avoiding microbial microleakage has not been proven. Purpose: To evaluate the differences of microbial microleakage using Enterococcus faecalis, in canals obturated with five different techniques: lateral, warm vertical, WaveOne® single cone, Guttacore®, and Hybrid-mixed condensation. Methods: 50 single-rooted human premolars extracted for orthodontic reasons were biomechanical prepared with primary file of WaveOne® system. Teeth were divided into 5 groups using different obturation techniques: single cone with WaveOne® Primary, lateral condensation using 2 % gutta-percha cones, Guttacore® 30, warm vertical condensation using down packing in a WaveOne® Primary cone and backfill with alpha gutapercha of Beefill®, and the hybrid mixed condensation modifying the lateral condensation with heat and a backfill using Beefill®. Enterococcus faecalis was inoculated in the coronal third and apices were immersed in brain heart infusion broth with phenol red incubated at 37 °C for 12 weeks. Microfiltration was determined with color change and turbidity of the medium. Specimens were observed by scanning electron microscopy. Results: Only 11 teeth (22 %) were positive for leakage. 46 % with single cone, 30 % with Guttacore®, 20 % with lateral condensation, 10 % with warm vertical condensation and no microleakage was found for Hybrid-Mixed Technique over the period of 12 weeks of study. Conclusion: Hybrid Mixed Technique showed to be the most efficient technique to get three-dimensional seal and prevent microbial contamination of canals in the endodontic therapy. Antecedentes: La técnica de obturación de conductos radiculares denominada “condensación híbrida-mixta”, combina las ventajas de la técnica lateral (en frío) con la técnica termoplastificada (con calor). Hasta el momento, su efectividad de sellado no ha sido estudiada Propósito: Evaluar la capacidad de sellado de conductos radiculares obturados con técnicas de: condensación lateral, condensación vertical, cono único, con portador-Guttacore® y condensación híbrida-mixta. Métodos: 50 dientes premolares extraídos por indicación ortodóntica y preparados con lima Primary de WaveOne® fueron distribuidos en 5 grupos y obturados con: cono único no estandarizado Primary de WaveOne®, condensación lateral utilizando conos de gutapercha al 2 %, obturadores calib