Muerte celular inducida por condiciones ambientales adversas en Calibrachoa parviflora (Petunia)
It has been previously reported that extreme environmental conditions such as high temperatures, ultraviolet light and the greenhouse effect, alter the rate of growth in plants. In this study we investigated the acute effect of two extreme environmental conditions: nutrient deprivation and soil acid...
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Veröffentlicht in: | Avances en Biomedicina 2012, Vol.1 (1), p.9-17 |
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Zusammenfassung: | It has been previously reported that extreme environmental conditions such as high temperatures, ultraviolet light and the
greenhouse effect, alter the rate of growth in plants. In this study we investigated the acute effect of two extreme
environmental conditions: nutrient deprivation and soil acidification with sulfuric acid, a compound commonly deposited in
the soil, as a result of acid rain, on the rate of death and cell proliferation in petunias (Calibrachoa parviflora). For this
purpose, a group of petunias was subjected to three different conditions: group A or control, kept in optimum conditions
and nutrient supply, group B in the absence of nutrients and natural light and group C, exposed to water acidified with
sulfuric acid. In continuous observations for up to 72 hours, samples from petals, leaves and stems of each plant were
analyzed and after nuclei extraction; these were treated with propidium iodide, a DNA intercalator compound, and
analyzed by flow cytometry and fluorescence microscopy. The results show that both extreme conditions generate an
imbalance in the process of death/survival of petunias, but unlike plants from group B, plants in group C, did not increase
their rate of cell proliferation. These results suggest that plants growing in acid soils lose their ability to compensate the
stress signal generated as a result of acute exposure to toxic concentrations of sulfuric acid.
Se ha descrito que condiciones ambientales extremas tales como altas temperaturas, luz ultravioleta y el efecto
invernadero, alteran la tasa de crecimiento de las plantas. En este trabajo se investigó el efecto agudo de dos condiciones
ambientales extremas tales como la limitación de nutrientes y la acidificación del suelo con ácido sulfúrico, un compuesto
comúnmente depositado en los suelos como consecuencia de la llamada lluvia ácida, sobre la tasa de muerte y proliferación
celular en petunias (Calibrachoa parviflora). Para tales efectos, grupos de petunias fueron sometidas a tres condiciones
diferentes: grupo A o control, mantenida en condiciones óptimas y de suministro de nutrientes, grupo B en ausencia de
nutrientes y de luz natural, y grupo C expuestas a agua acidificada con ácido sulfúrico. En un seguimiento hasta 72 horas, se
analizaron muestras de pétalos, hojas y tallos de cada una de las plantas y, posterior a la extracción de los núcleos, estos
fueron teñidos con ioduro de propidio, un agente intercalante del ADN, y analizados mediante citometría d |
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ISSN: | 2244-7881 |