Two new protist species, Trypanoplasma ojedae sp. n. (Mastigophora: Kinetoplastida) and Trichodina lascrucensis sp. n. (Ciliophora: Peritrichida) in a blenniid fish, Scartichthys viridis, from the coast of Chile

Two new protistan species were observed in a blenniid fish, Scartichthys viridis, inhabiting tide pools at Las Cruces, central Chile. Trypanoplasma ojedae sp. n. (Mastigophora: Kinetoplastida) in the blood is characterized by its small size (29.8 ± 5.0 x 5.1 +1.2 ìm), short anterior flagellum (4.9 ± 0.6 ìm), anterior located kinetoplast (2.4 ± 0.2) and a prominent undulating membrane. All of 18 fish were infected and 67% were parasitized on the gills by an unidentified piscicolid leech that harbored developing and infective biflagellated stages. Trichodina lascrucensis sp. n. (Ciliophora: Peritrichida) parasitized the gill fillaments of all fish. The ciliate is characterized by its body size (70.8 ± 8.4 ìm), adhesive disc (53 ± 5.4 ìm), denticular ring (33 ± 3.1 ìm), mean number of denticles (32.8 ± 3.0), mean number of denticular pins (9.5 ± 1.0) and ovoid micronucleus located near to one of the macronuclear arms. Since none of the two parasites was similar to any species described from marine tide pool habitats as well as from the Pacific Ocean, both are considered new species with the characteristics described herein. Dos protistas parasitos fueron observados en un pez blenido, Scartichthys viridis, que habita en pozas de mareas en Las Cruces, Chile central. En la sangre, Trypanoplasma ojedae sp. n. (Mastigophora: Kinetoplastida) se caracteriza por poseer pequeno tamano (29,8 } 5,0 x 5,1 }1,2 Êm), un corto flagelo anterior (4,9 } 0,6 Êm), situado anterior al kinetoplasto (2,4 } 0,2) y una prominente membrana ondulante. De 18 peces todos estaban infectados y el 67 % se encontraba parasitado en las branquias por una especie de sanguijuela no identificada de la familia Piscicolidae que albergaba estados en desarollo e infectantes del biflagelado. Trichodina lascrucensis sp. n. (Ciliophora: Peritrichida) parasita los filamentos branquiales de todos los peces. El ciliado se caracteriza por su tamano corporal (70,8 } 8,4 Êm), disco adhesivo (53 + 5,4 Êm), anillo denticular (33 } 3,1 Êm), numero promedio de denticulos (32,8 } 3,0), numero promedio de pins (9,5 } 1,0) forma de los denticulos y micronucleos ovoides localizados cerca del armamento macronuclear. Debido a que ninguno de los dos parasitos es similar a cualquier especie descrita para habitats de pozas de mareas del Oceano Pacifico, ambas son consideradas nuevas especies con las caracteristicas descritas aca.