Efecto de la acriflavina, formalina y glutaraldehído sobre la desinfección y la eclosión de los huevos del botete diana Sphoeroides annulatus

Three prophylactic treatments using three dosages each were used as surface disinfectants in bullseye puffer Sphoeroides annulatus eggs to study their effect on bacterial load reduction and hatching rate. Eggs were treated with proteolytic enzyme to remove stickiness. Tested treatments were: acriflavine (A) at a concentration of 2.5, 5 and 10 ppm for 1 min; formalin (F) at 10, 50 and 100 ppm for 30 min and glutaraldehyde (G) at 50, 100 and 200 ppm for 5 min, as well as a control group with no treatment (C), using three replicates per treatment. After disinfection, an egg sample from each replicate was inoculated in duplicate on TCBS and marine agar culture media to assess bacterial growth (CFU mL-1). The eggs were incubated and their hatching rate determined. Culture media tested negative for Vibrio spp. in all treatments. However, the growth of heterotrophic bacteria was present in all experimental groups; G-100 showed a significant reduction in CFU (4.00 ± 2.8 x 102 mL-1), whereas A-2.5 and F-50 had significantly higher CFU values (3.45 ± 0.4 x 104 and 5.73 ± 0.3 x 104 mL-1 respectively). By not disinfecting the eggs, a higher hatching rate was obtained (96.1 ± 6.2%), however relatively high CFU counts (1.22 ± 0.1 x 104 mL-1) were observed in this treatment, while the lowest hatching rate was found in eggs disinfected with A-5 (12.3 ± 6.3%). Thus, the use of stickiness removal procedures prior to incubation of bullseye puffer eggs seems to be enough to produce good hatching rates. Se evaluaron tres tratamientos profilácticos, a tres dosis diferentes cada uno, para desinfectar huevos de botete diana Sphoeroides annulatus y determinar su efecto en la reducción de la carga bacteriana y el porcentaje de eclosión. Los huevos fueron tratados con enzima proteolítica para desgomarlos. Los tratamientos se realizaron por triplicado: acriflavina (A) a concentraciones de 2,5, 5, y 10 ppm por 1 min, formalina (F) a 10, 50 y 100 ppm por 30 min y glutaraldehído (G) a 50, 100 y 200 ppm por 5 min y un grupo control sin tratamiento (C). Se inoculó por duplicado una muestra de huevos de cada réplica en agar TCBS y marino para evaluar el crecimiento bacteriano (UFC mL-1); los huevos se incubaron y se determinó el porcentaje de eclosión. Los resultados del recuento en todos los tratamientos fueron negativos para Vibrio spp.; sin embargo, las bacterias heterótrofas se presentaron en todos los tratamientos; en G-100 ppm se observó la menor cantidad de UFC (4,00 ± 2,8 x 102 mL-