Supplementary Figure 1, Supplementary Figure 2

Supplementary Figure 1. Optical contrast phase images of 2D neuronal network under static (A) and dynamic (B) conditions perfused with variable flows according to protocol 1 at DIV 25. (C-D) 2D neuronal cultures, labeled for Tubulin βIII (green) and GFAP (red), at DIV 25, under static (C) and dynamic (D) conditions. Scale bar: 50 μm. Supplementary Figure 2. Neurons grown for 25 DIV under static (A) and dynamic conditions perfused with variable flows according to protocol 1 (B), marked for Tubulin βIII (green), PSD-95 (red), and Dapi (blue). Scale bar: 10 μm.