MOESM2 of SCP2-mediated cholesterol membrane trafficking promotes the growth of pituitary adenomas via Hedgehog signaling activation

Additional file 2: Figure S2. Inhibition efficiency assay and identification of primary human GH-producing PA cells. A. The inhibitory effect of different concentrations of itraconazole on SCP2 expression was assessed in GH3 cells by Western blotting. For subsequent experiments, 10 μM itraconazole was used, according to the expression levels of SCP2. B. The inhibitory effect of different concentrations of vismodegib on the Hh signaling pathway was assessed in GH3 cells by Western blotting. For subsequent experiments, 50 μM vismodegib was used, according to the expression levels of GLI1. C. SMO mRNA levels were measured in GH3 cells by RT-qPCR after transfection with shRNA. SMO-RNAi-2 and SMO-RNAi-3 were used for subsequent experiments, according to the expression levels of SMO (n = 3, ± SEM). D. Agonistic effects of different concentrations of forskolin on PKA expression were assessed in GH3 cells by Western blotting. For subsequent experiments, 25 μM forskolin was used, according to the expression levels of PKA. F. Cells derived from the primary GH-producing PA sample were identified by the presence of human GH. Green signal, GH staining; blue signal, DAPI nuclear staining. Scale bar, 50 μm. An unpaired t-test was used to assess statistical significance. *P