MOESM6 of Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells

Additional file 6: Figure S5. Differential expression of neutral and sialyl sugar chains in normal-phase HPLC analysis of the N-linked oligosaccharide patterns of human defuse large B cell lymphoma (DLBCL) cells. (Aâ B) Neutral (A) and sialyl (B) sugar chains were detected in non-CNS lymphomas, including A4/FUK, HBL1, and OYB. Intensities were evaluated with the percent (%) area of peaks in each fraction eluting at retention times of M4, M5, M6, M7, M8, and M9. The peak numbers refer to the oligosaccharide structures in Additional file 5: Figure S4. (C) Summary for expression changes of neutral and sialyl sugar chains among A4/FUK, HBL1, and OYB as non-CNS lymphomas. Color configuration indicates high (red) to low (green). S0: non-sialyl sugar chain, Neu: Neuraminidase-treated sugar chains. (D) Percent area (%) of peaks in each fraction eluting at retention times of sialyl sugar chains form A4/FUK (red), HBL1 (blue), and OYB (green). (E) Summary of percent area of sialyl sugar chains among A4/FUK, HBL1, and OYB. Color configuration indicates high (red) to low (black). NA, not applicable.