MOESM5 of CD171- and GD2-specific CAR-T cells potently target retinoblastoma cells in preclinical in vitro testing

Additional file 5: Figure S4. B-cell lymphoma cell line NALM-6 serves as negative control. A. NALM-6 cells do not express antigens CD171 and GD2 as analyzed by flow cytometry. IFNG and IL2 release of CD171- (B) and GD2-specific CAR-T cells (C) following a 24-h co-culture at a 2:1 E:T ratio with NALM-6 cells compared to RBL15 retinoblastoma cells (mean ± SD, n = 1 in technical triplicates). D. Cytotoxicity of CD171-and GD2-specific CAR-T cells is displayed in comparison to mock-transduced T cells. Cytotoxicity was measured by a luciferase-based killing assay following a 24 h co-culture at a 2:1 E:T ratio compared to RBL15 cells (mean ± SD, n = 1 in technical triplicates). Color-coding is the same as used in B. (JPG 128 kb)