Quantitative phase microscopy images of spherical droplets, beads, and cells

Example data sets for quantitative phase imaging.These data were used by Müller et al. [1] to demonstrate theaccurate retrieval of size and refractive index for sphericalobjects by means of a two-dimensional phase data fittingalgorithm.Abbreviations: - DHM: digital holographic microscopy - FUS: fused in sarcoma (RNA-binding protein) - HL60: name of a human leukemia cell line - QLSI: quadri-wave lateral shearing interferometry - PAA: Polyacrylamide (hydrogel) - PBS: phosphate buffered saline (buffer medium)The data are related to [1] according to the following scheme:Figure 4: a-c) SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07 Silicone oil droplet measured in PBS using QLSI d-f) SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5 FUS protein droplet measured in egg extract using QLSI (included in fig. 5a) g-j) SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23 PAA hydrogel bead (referred to as "type A" in [1]) measured in PBS using QLSI (included in fig. 5b) k-m) DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11 PAA hydrogel bead (referred to as "type B" in [1]) measured in PBS using DHM n-q) QLSI_2015-11-09_16-03-52_HL60 HL60 cells measured in PBS using QLSI (included in fig. 5c) r-s) DHM_2015-11-09_16-48-45_HL60 HL60 cells measured in PBS using DHM (included in fig. 5d)Figure 5: a) QLSI_2016-04-29_FUS-droplet-780-5 Time lapse measurement of a FUS protein droplet measured in egg extract using QLSI b) QLSI_2016-07-05_PAA-beads-S6-A PAA hydrogel beads (referred to as "type A" in [1]) measured in PBS using QLSI c) QLSI_2015-11-09_HL60 HL60 cells measured in PBS using QLSI d) DHM_2015-11-09_HL60 HL60 cells measured in PBS using DHMThe data are stored in different file formats: - .tif: digital holography data or (!) SID4Bio (Phasics S.A.) data - .h5: HDF5-based qpimage [2] file format - .zip: each ZIP file contains several .tif files - .cfg: meta data and analysis recipe for each data set, stored as a DryMass [3] profileThe phase data can be extracted with DryMass [3] and qpformat [4],e.g. using the command-line interface of DryMass: dm_analyze_sphere --profile DHM_2015-11-09_HL60.cfg DHM_2015-11-09_HL60.zipFor more information and a detailed analysis, please refer to [1].License: CC0 (https://creativecommons.org/publicdomain/zero/1.0/)SHA256 sums:cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_16-48-45_HL60.cfg04eaf59da61934486385f42600aec0a2ffb3f71c93c2b6b6f247ac237088eefb DHM_2015-11-09_16-48-45_HL60.tifcac55752e36ddf3a1960dc8fe5c1514455da1fb8