Regulation of chimeric TH–INS1 mRNA expression

Copyright information:Taken from "The regulated expression of chimeric tyrosine hydroxylase–insulin transcripts during early development"Nucleic Acids Research 2006;34(12):3455-3464.Published online 13 Jul 2006PMCID:PMC1524912.© 2006 The Author(s) () Photos of chick embryos at different stages (st). Stage 4 corresponds to gastrulation (≈18 h of incubation), stage 8 to early neurulation (≈24 h of incubation) and stage 10 to the end of neurulation (≈36 h of incubation). () Quantitative RT–PCR of RNA from chick embryos at the developmental stages indicated. The levels of each transcript were normalized to the levels of GAPDH mRNA. The results represent the mean ± SD. * < 0.05; ** < 0.01; when compared to stage 4 calculated with the C Dunnett's test comparing all experimental groups. (–) Quantitative RT–PCR on RNA from stage 8 embryos, from an E13 (PE13) pancreas and from the of an E18 embryonic brain (SNE18). The results represent the mean ± SD. H, heart; HF, head fold; OV, optic vesicle; PS, primitive streak; and S, somites.