Experimental design, consisting of 30 two-dye CATMA v2

Copyright information:Taken from "Genome-wide screening for -regulatory variation using a classical diallel crossing scheme"Nucleic Acids Research 2006;34(13):3677-3686.Published online 2 Aug 2006PMCID:PMC1540733.© 2006 The Author(s)2 microarray () experiments to examine transcript levels in RNA samples collected from a diallel experiment in with 5 parental lines and 10 F hybrids. The five hybridized parental lines were the homozygous accessions Col, L, Cvi, Ws and C24. F hybrids were obtained by making all pairwise crosses between the five parental lines. The 10 hybrid samples hybridized consisted of pooled progeny from reciprocal crosses. The microarray contained 22 494 unique GSTs from and is represented by an arrow, connecting the two sampled genotypes hybridized to it. The samples at the tail and head of each arrow were labeled with Cy3 and Cy5, respectively. The two replicates are represented by differently colored arrows.