Effect of indomethacin on IL-1 receptor antagonist (IL-1Ra) secretion in human synovial fibroblasts and articular chondrocytes

Copyright information:Taken from "The active metabolite of leflunomide, A77 1726, increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes"Arthritis Research & Therapy 2004;6(3):R181-R189.Published online 19 Feb 2004PMCID:PMC416438.Copyright © 2004 Palmer et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Human osteoarthritis (OA) synovial fibroblasts (passage 1), de-differentiated human OA articular chondrocytes (passage 2), and freshly isolated human OA articular chondrocytes were stimulated or not (control; white bars) with 1 ng/ml IL-1β (hatched bars), the combination of IL-1β and 5 μg/ml indomethacin (gray bars), or the combination of IL-1β and 100 μmol/l A77 1726 (black bars) for 48 hours. Indomethacin or A77 1726 was added 2 hours before stimulation with IL-1β. IL-1Ra concentrations in culture supernatants were measured by ELISA. Results are presented as means ± SEM of three determinations in a representative experiment. Similar results were obtained with cells from six different donors (six OA samples) for synovial fibroblasts, seven different donors (seven OA samples) for de-differentiated chondrocytes and six different donors (three normal and three OA samples) for freshly isolated chondrocytes. *< 0.05 versus control; < 0.05 versus IL-1β alone; **< 0.001 versus IL-1β alone.