The and insulin genes are unique in the chicken genome

Copyright information:Taken from "The regulated expression of chimeric tyrosine hydroxylase–insulin transcripts during early development"Nucleic Acids Research 2006;34(12):3455-3464.Published online 13 Jul 2006PMCID:PMC1524912.© 2006 The Author(s) () Southern blot analysis of genomic DNA digested with the restriction enzymes indicated and hybridized with either a TH cDNA probe or a TH genomic probe. BamHI generates bands of 5000 and 3880 bp; EcoRI generates bands of 12 020 and 5539 bp; HindIII generates a band of 7411 bp. () PCR of the -insulin intergenic region. The first PCR was performed with the P13 (located in the 3′-UTR) and P14 (located in the insulin 5′-flanking region); for the second PCR, the P13 and P15 primers (located in the insulin 5′-flanking region) were used.