(A) The double-disrupted strains were transformed with the respective plasmid-borne wt or mutant genes

Growth was analyzed by spotting transformants in 10-fold serial dilutions on 5-FOA–containing plates at the indicated temperature for 5 d or on synthetic dextrose complete–Leu-Trp for 3 d (Δ, , and ). No growth indicates synthetic lethality. (B) Schematic representation of the genetic network between and factors involved in transcription-coupled mRNA export. Arrows to gray components indicate synthetic lethality/enhancement, and proteins depicted in white are genetically not linked to .Copyright information:Taken from "The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression"The Journal of Cell Biology 2008;182(5):897-910.Published online 8 Sep 2008PMCID:PMC2528585.