Modulation of chloride homeostasis by inflammatory mediators in dorsal root ganglion neurons-0

Mmunfluorescence of calcitonin gene-related peptide (CGRP) and substance P (SP) was evaluated at the indicated times after start of the treatment. The relative number of neuropeptide-expressing neurons was determined in test DRGs and in contralateral control DRGs, and the percent change of positive cells, Δ NP = (NP-NP)/NP·100 [%], is given for each time. Indicated significance levels are p ≤ 0.05 (*) or p ≤ 0.01 (**). (B) Cryosections of a control DRG and the treated contralateral test DRG stained with NKCC1 antibodies. The red circles illustrate the area from which the mean fluorescence intensity was obtained. Background subtraction removed most of the pericellular fluorescence as well as the fluorescence originating from the nucleus. All cell sections with intact perimeter were evaluated. (C) Distribution of fluorescence intensities recorded from 3 control DRGs and 3 contralateral test DRGs illustrate a shift to higher intensities after a 3-hr treatment with inflammatory mediators. Solid lines are Gaussian fits to the data.Copyright information:Taken from "Modulation of chloride homeostasis by inflammatory mediators in dorsal root ganglion neurons"http://www.molecularpain.com/content/4/1/32Molecular Pain 2008;4():32-32.Published online 12 Aug 2008PMCID:PMC2526990.