Functional and biochemical characterization of the 20S proteasome in a yeast temperature-sensitive mutant, -2

20S proteasome, and 10 μM of peptidyl substrates (: Suc-LLVY-MCA for chymotrypsin-like activity; : Z-LLE-MCA for PGPH activity; : Boc-LRR-MCA for trypsin-like activity) at 37°C for 1 h. Reactions were started and stopped as described in methods. Symbols represent the wild-type strain (○) and the mutant (●). Values are means ± SD for three independent experiments.Copyright information:Taken from "Functional and biochemical characterization of the 20S proteasome in a yeast temperature-sensitive mutant, "http://www.biomedcentral.com/1471-2091/9/20BMC Biochemistry 2008;9():20-20.Published online 21 Jul 2008PMCID:PMC2515314.