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Copyright information:Taken from "Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in "Nucleic Acids Research 2006;34(11):3361-3369.Published online 5 Jul 2006PMCID:PMC1488887.© 2006 The Author(s) () β-Galactosidase activity levels of recombinant DH5...

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Hauptverfasser: Prajna R. Kulkarni, Xiaohui Cui, Williams, Joshua W., Stevens, Ann M., Kulkarni, Rahul V.
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Sprache:eng
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Zusammenfassung:Copyright information:Taken from "Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in "Nucleic Acids Research 2006;34(11):3361-3369.Published online 5 Jul 2006PMCID:PMC1488887.© 2006 The Author(s) () β-Galactosidase activity levels of recombinant DH5α strains encoding or transcriptional fusions in pSP417. Background levels of β-galactosidase produced from the negative control pSP417 were 0.063 ± 0.004 RLU. Error bars represent the standard deviation of assays performed in triplicate from three independent samples. () Northern blot analysis of the rate of transcription of and in ES114 grown to different OD values as indicated using sequences as a probe. Identical results were obtained when sequences were used as a probe (data not shown). The blot shown is representative of two independent experiments. The migration of RNA size standards is indicated on the right.
DOI:10.6084/m9.figshare.8687