(A–F) Identical exposures of GLP-1 protein staining after RNAi with empty vector (control RNAi; A and B), (C and D), or (E and F)

In all three RNAi conditions, GLP-1 expression was high in the distal tip (not depicted) but was repressed normally in meiotic region of the distal arm (A, C, and E). In (D) and (F) oocytes, GLP-1 staining was equivalent or greater than distal tip staining on the same slide (high GLP-1 staining). Outlines denote the gonad surface. (G) The number of gonads with high GLP-1 staining in oocytes was scored blind in RNAi, homozygotes and wild-type () gonads. (H) mRNA from equal numbers of wild-type (N2) or hermaphrodites was assayed by semiquantitative RT-PCR. Primers distinguished spliced (shown) from unspliced mRNA and DNA products, which were not detected (not depicted). (top) Each lane shows cDNA from one worm equivalent of total RNA; mRNA levels were similar in both samples (not depicted). (bottom) Changes in RT-PCR product with cDNA input amount suggest that mutant and wild-type RNA levels were equivalent or differed by less than twofold.Copyright information:Taken from "Maternal mRNAs are regulated by diverse P body–related mRNP granules during early development"The Journal of Cell Biology 2008;182(3):559-572.Published online 11 Aug 2008PMCID:PMC2500140.