The cerebral cortex of a control brain (A) shows a diffuse extracellular pattern, most prominent in the glia limitans

Hydrocephalic brains appear the same (not shown). Omission of the primary antibody is associated with no labeling (B). Normal white matter (C, arrow) is less intensely labeled. White matter in hydrocephalic brains (D, arrow) is atrophic and the residual tissue is more intensely labeled. Scale bar = 100 μm. Bar graph shows densitometric analysis of immunolabeling (arbitrary units) in white matter of control (C) and hydrocephalic (H) rats. The hydrocephalus-related increase is significant only in the 3-week rats (*= 0.0007 ANOVA, Bonferroni-Dunn; all groups n = 4). No significant changes were observed in any other location.Copyright information:Taken from "Effect of hydrocephalus on rat brain extracellular compartment"http://www.cerebrospinalfluidresearch.com/content/5/1/12Cerebrospinal Fluid Research 2008;5():12-12.Published online 10 Jul 2008PMCID:PMC2488327.