Schematic of vRNA production

Copyright information:Taken from "A universal reference sample derived from clone vector for improved detection of differential gene expression"BMC Genomics 2006;7():109-109.Published online 5 May 2006PMCID:PMC1475850.Copyright © 2006 Khan et al; licensee BioMed Central Ltd. (a) The parental EST clone vector (pT7T3D-pac) is used as a template for PCR with GF200 primers. (b) The PCR product is further amplified and converted to aRNA via an in vitro transcription reaction. (c) The aRNA is reverse transcribed and incorporated with amino allyl dUTP creating vcDNA. (d) The vcDNA is coupled with Cy3 dye. (e) The product of eight labeling reactions are combined (20 uL) and diluted to 125 uL.