A semi-automated multiplex high-throughput assay for measuring IgG antibodies against erythrocyte membrane protein 1 (PfEMP1) domains in small volumes of plasma-1

Ma samples from 60 individuals living in the three Tanzania villages Mgome (high malaria transmission), Ubiri (medium malaria transmission) and Magamba (low malaria transmission). The samples for both assay platforms were diluted 1:80 and results were expressed as median fluorescent intensity for the bead-based assay and arbitrary ELISA Units for ELISA. The Spearman's rank correlation coefficients (Rho) were ≥ 0.86, (P < 0.0001). Note: The graphs have different scales. MFI: median fluorescent intensity and EU: ELISA units.Copyright information:Taken from "A semi-automated multiplex high-throughput assay for measuring IgG antibodies against erythrocyte membrane protein 1 (PfEMP1) domains in small volumes of plasma"http://www.malariajournal.com/content/7/1/108Malaria Journal 2008;7():108-108.Published online 12 Jun 2008PMCID:PMC2435541.