Time course of statin dependent effects on proliferation and AML cell viability

The time dependence of lovastatin (LST) induced effects on proliferation and apoptosis were determined in U937 or HL60 cells. Treated samples were compared with that of untreated cells. (> 3, one example is shown for each assay.) (A) Cells counts by day of culture, at various concentrations. Cell number was determined using a Coulter counter. Shown are total cell counts. At lower concentrations (i.e. 5 15 mM), differences between treated and untreated cells are best seen after at least 4 days of statin exposure. (B) Time course of changes in absorbance (MTS assay). Cell proliferation was measured using an MTS-like colorimetric assay that reflects cellular metabolism. (C) Effects of statins during the first 2 days of culture. Cells were plated at a concentration of 10,000 cells per well in order to look at the effects of lovastatin on proliferation during the first 2 days of drug exposure. These results show that lovastatin did not markedly affect proliferation during the first 2 days of statin exposure, especially at the lower concentrations studied. (D) Percentage of viable cells, by apoptosis, after lovastatin exposure, by day of culture (HL60 cells). Percentage of cells, by day, that were negative for both PI and Annexin. (E) Viable cell number. Using the same data set as shown in 1D, the number of viable cells was determined for each day of treatment by multiplying the number of cells as determined the Coulter counter by the percentage of cells negative for both annexin and PI. In each assay, the cytotoxic effects of concentrations of statins in the clinically relevant range (i.e. Copyright information:Taken from "Statins induce lethal effects in acute myeloblastic lymphoma cells within 72 hours"Leukemia & Lymphoma 2008;49(2):322-330.Published online Jan 2008PMCID:PMC2430172.