Additional file 5: of Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition

Figure S5. . Piperacillin treatment during transition to stationary phase and its impact on persister formation. Cell cultures were treated with 200 µg/mL piperacillin (PIP) at t= 4, 5 or 6 h. Cells in control culture were treated with an equal volume of water (untreated). Cells were fixed at t = 24 h before washes, and imaged using a brightfield microscope (A). Cell cultures were washed to remove chemicals and diluted in fresh LB containing 200 µg/mL ampicillin or 5 µg/mL ofloxacin. CFU levels were monitored at the indicated time points. Data denoted as survival fraction (B and D) and CFU/mL (C and E). * p