Epigenetic changes within the promoter region of the gene in ovarian tumors-4

Ncers and regulator binding sites: HRE = hypoxia response element; B2 = enhancer κB2; B1 = enhancer κB1; ISRE = interferon sequence responsive element; W/S= W/S box; X1 = conserved X1 regulatory box; X2 = X2 box; CAAT = CCAAT box; TATA = TATA box; ex1 = exon 1; Met-F and Met-R = forward (F) and reverse (R) primer binding sites. Bisulfite genomic sequencing of 19 CpG dinucleotides of the region from -450 to ATG. Individual CpG dinulcotides are depicted as circles. Each row of circles represents an individual sequenced clone, either untreated (PBS) or treated with 50 μM 5-aza-dC (open circle = 100% unmethylated; filled circle = 100% methylated; %MET = percentage of methylation of each individual clone; %CONV = efficiency of sodium bisulfite treatment).Copyright information:Taken from "Epigenetic changes within the promoter region of the gene in ovarian tumors"http://www.molecular-cancer.com/content/7/1/43Molecular Cancer 2008;7():43-43.Published online 22 May 2008PMCID:PMC2429914.