Additional file 1: of Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1
Figure S1. Effects of pirfenidone on TGF-β1-mediated regulators of the COX2)/ PGE2 pathway in fibroblasts. Subconfluent HFL-1 cells were cultured in SF-DMEM for 24 h and then incubated in the presence or absence of TGF-β1 (10 pM) and pirfenidone (100 ng/mL) for 48 h. Proteins were extracted and subj...
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| creator | Jin, Jin Togo, Shinsaku Kadoya, Kotaro Miniwan Tulafu Namba, Yukiko Iwai, Moe Watanabe, Junko Nagahama, Kumi Okabe, Takahiro Moulid Hidayat Kodama, Yuzo Hideya Kitamura Ogura, Takashi Norikazu Kitamura Ikeo, Kazuho Sasaki, Shinichi Tominaga, Shigeru Takahashi, Kazuhisa |
| description | Figure S1. Effects of pirfenidone on TGF-β1-mediated regulators of the COX2)/ PGE2 pathway in fibroblasts. Subconfluent HFL-1 cells were cultured in SF-DMEM for 24 h and then incubated in the presence or absence of TGF-β1 (10 pM) and pirfenidone (100 ng/mL) for 48 h. Proteins were extracted and subjected to western blot analysis of COX2. Media were harvested from monolayer culture and evaluated for PGE2 by immunoassay. Release of PGE2 (A) in HFL-1 monolayer cultures and western blot analysis of COX2 (69 kDa) (B). Bar figure vertical axis: protein expression relative to β-actin. Horizontal axis: conditions. Values represent means ± SEMs of at least three independent experiments. (TIF 579 kb) |
| doi_str_mv | 10.6084/m9.figshare.8261723 |
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Effects of pirfenidone on TGF-β1-mediated regulators of the COX2)/ PGE2 pathway in fibroblasts. Subconfluent HFL-1 cells were cultured in SF-DMEM for 24 h and then incubated in the presence or absence of TGF-β1 (10 pM) and pirfenidone (100 ng/mL) for 48 h. Proteins were extracted and subjected to western blot analysis of COX2. Media were harvested from monolayer culture and evaluated for PGE2 by immunoassay. Release of PGE2 (A) in HFL-1 monolayer cultures and western blot analysis of COX2 (69 kDa) (B). Bar figure vertical axis: protein expression relative to β-actin. Horizontal axis: conditions. Values represent means ± SEMs of at least three independent experiments. 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Effects of pirfenidone on TGF-β1-mediated regulators of the COX2)/ PGE2 pathway in fibroblasts. Subconfluent HFL-1 cells were cultured in SF-DMEM for 24 h and then incubated in the presence or absence of TGF-β1 (10 pM) and pirfenidone (100 ng/mL) for 48 h. Proteins were extracted and subjected to western blot analysis of COX2. Media were harvested from monolayer culture and evaluated for PGE2 by immunoassay. Release of PGE2 (A) in HFL-1 monolayer cultures and western blot analysis of COX2 (69 kDa) (B). Bar figure vertical axis: protein expression relative to β-actin. Horizontal axis: conditions. Values represent means ± SEMs of at least three independent experiments. 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Effects of pirfenidone on TGF-β1-mediated regulators of the COX2)/ PGE2 pathway in fibroblasts. Subconfluent HFL-1 cells were cultured in SF-DMEM for 24 h and then incubated in the presence or absence of TGF-β1 (10 pM) and pirfenidone (100 ng/mL) for 48 h. Proteins were extracted and subjected to western blot analysis of COX2. Media were harvested from monolayer culture and evaluated for PGE2 by immunoassay. Release of PGE2 (A) in HFL-1 monolayer cultures and western blot analysis of COX2 (69 kDa) (B). Bar figure vertical axis: protein expression relative to β-actin. Horizontal axis: conditions. Values represent means ± SEMs of at least three independent experiments. (TIF 579 kb)</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.8261723</doi><oa>free_for_read</oa></addata></record> |
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| title | Additional file 1: of Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1 |
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