The relative amounts of (A) and (B) transcripts from S3, R3, and S3∷R measured by qrtRT-PCR

Error bars show the SEM of assays performed in triplicate. P < 0.005 for differences in and mRNA levels in R3 and S3∷R versus S3 using the Student's test. (C) Western blot analysis of SiaA and RecA in whole-cell lysates. The strains are indicated above each lane, and the sizes of the proteins (in kD) are shown. (D) Expression of the polysialic capsule detected by FACS. Bacteria were incubated with polyclonal antisera raised against the MCC vaccine (black, capsule-negative strain R3Δ; blue, S3; red, S3∷R; purple, R3). (E) Relative amount of capsule detected by FACS. Results are shown as the MFI (calculated as the geometric mean multiplied by the percentage of positive cells). Error bars show the SEM. P < 0.005 for both strains with increased resistance versus S3 using the Student's test.Copyright information:Taken from "A generic mechanism in for enhanced resistance against bactericidal antibodies"The Journal of Experimental Medicine 2008;205(6):1423-1434.Published online 9 Jun 2008PMCID:PMC2413038.