Additional file 5: of Subtractive hybridization-assisted screening and characterization of genes involved in the rice-Magnaporthe oryzae interaction

Figure S4. Genes unfit for DSH. No subtractive effects were observed for constitutive genes MGG_01160 and MGG_04467, and cDNAs of the upregulated gene OsPGIP1 were lost after DSH treatment. Gene-specific primers were used for PCR analysis of DSH and Non-DSH treated samples. Aliquots of PCR reaction mixture were taken at 20, 25, 30, and 35â cycles of amplification and the products were analyzed on 1% agarose gels as indicated. Lane M, DNA ladder (Generay. Code No. 1705G22). (TIF 7701 kb)