Expression of zebrafish in pancreas is regulated by two enhancers containing highly conserved -elements bound by PDX1, PBX and PREP factors-4

For the constructs, the different regions to test were fused to the heterologous promoter. The arrow indicates the transcriptional start site at the . Fusion constructs (AB-and C-) were made by PCR using specific primers (see materials and methods for sequences). These DNA constructs were injected into zebrafish embryos using Tol2 mediated transgenesis and GFP transient expression was analysed at 24, 48 and 75 hours of development. Transient GFP expression in 75 and 48 hpf embryos injected with the full ABC-and the C-constructs. All the embryos are presented in dorsal views with anterior on the left. Abbreviations: , pancreas.Copyright information:Taken from "Expression of zebrafish in pancreas is regulated by two enhancers containing highly conserved -elements bound by PDX1, PBX and PREP factors"http://www.biomedcentral.com/1471-213X/8/53BMC Developmental Biology 2008;8():53-53.Published online 16 May 2008PMCID:PMC2409314.