Expression of zebrafish in pancreas is regulated by two enhancers containing highly conserved -elements bound by PDX1, PBX and PREP factors-3

deletion constructs were made by enzymatic digestion at the sites indicated. A, B and C conserved regions of approximately 100-bp long are indicated by blue boxes. These DNA constructs were injected into zebrafish embryos and DSRED (-I transgenesis) or GFP (Tol2 transgenesis) transient expression was analysed at 24, 48 and 75 hours of development. The percentage of embryos expressing DSRED/GFP in the retina, the pancreas and the telencephalon versus the total number of embryos expressing DSRED/GFP (n) is indicated in brackets; the number of DSRED/GFP-expressing cells in each tissue (from +++ to +) is shown in the table. For the stable transgenic lines, n is the number of different transgenic founders obtained, and the level of DSRED expression is indicated (from +++ to -). Transient GFP expression in 75 and 48 hpf embryos injected with the full PO (FullP0), the A-deleted (P0-A) and the C-deleted (PO-C) constructs using Tol2-mediated transgenesis. DSRED expression in stable transgenic embryos at 48 hpf harboring the full P0 construct, the A-deleted construct or the B-deleted construct after -I transgenesis. Abbreviations: , I; , I; , I; , I; , pancreas; , retina; , telencephalon. All the embryos are presented in dorsal views with anterior on the left.Copyright information:Taken from "Expression of zebrafish in pancreas is regulated by two enhancers containing highly conserved -elements bound by PDX1, PBX and PREP factors"http://www.biomedcentral.com/1471-213X/8/53BMC Developmental Biology 2008;8():53-53.Published online 16 May 2008PMCID:PMC2409314.