Additional file 2: of Single-cell RNA sequencing reveals the impact of chromosomal instability on glioblastoma cancer stem cells

Validation of estimated copy number in TCGA GBM dataset. a, Heatmap of estimated copy number (ECN) of all chromosomes (columns) in GBM cancer tissue and adjacent normal tissue (rows). On the scale, ECN = 0 indicates diploid gene expression levels. b, Quantification of chromosomal instability in tumor tissue and adjacent normal tissue. Bar, median; box 25th to 75th percentile; whiskers, minimum and maximum. P = 2E-4, Mann-Whitney U test comparing tumor and normal tissue. c, Correlation between ECN and SNP-array measured copy number using patient sample TCGA-12-0619-01A. Spearman correlation coefficient = 0.71. d, The distribution of the correlation coefficient across samples in the glioma dataset. The dashed line indicates the median correlation. (TIF 5482 kb)