Additional file 5: of Efficient disruption of bcr-abl gene by CRISPR RNA-guided FokI nucleases depresses the oncogenesis of chronic myeloid leukemia cells

Figure S4. RFNs have hardly any effect on the proliferation and apoptosis of bcr-abl negative CD34+ cells. The bcr-abl negative CD34+ cells were isolated from individuals diagnosed with leukocytosis or anemia, and transfected with gRNA-17 plus donor or RFNs plus donor. (a)-(c) Cell viability of norm...

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Hauptverfasser: Luo, Zhenhong, Gao, Miao, Huang, Ningshu, Wang, Xin, Yang, Zesong, Yang, Hao, Huang, Zhenglan, Feng, Wenli
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Sprache:eng
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Zusammenfassung:Figure S4. RFNs have hardly any effect on the proliferation and apoptosis of bcr-abl negative CD34+ cells. The bcr-abl negative CD34+ cells were isolated from individuals diagnosed with leukocytosis or anemia, and transfected with gRNA-17 plus donor or RFNs plus donor. (a)-(c) Cell viability of normal CD34+ cells was detected by CCK-8 assay. (d) Apoptotic proportion of normal CD34+ cells was determined by flow cytometry. (TIF 413 kb)
DOI:10.6084/m9.figshare.8197184