Additional file 5: of Drosophila Netrin-B controls mushroom body axon extension and regulates courtship-associated learning and memory of a Drosophila fragile X syndrome model

Figure S4. NetB from the MB cells doesn’t interact with the Fra and Unc5 of the same cells. a. Knock-down of Fra and Unc-5 with NetB overexpression in the MB cells failed to ameliorate the defects of overextended α and β lobes (OK107 > UAS-NetB, n = 28; UAS-NetB/+; UAS-Fra RNAi/+; OK107-Gal4/+, n = 30; UAS-NetB/+; UAS-Unc-5 RNAi/+; OK107-Gal4/+, n = 28). b. Knock-down of Fra and Unc-5 with OK107-Gal4 displayed normal α/β lobes with normal length. Knock-down of Fra by a glia specific driver repo-Gal4, the MBs showed normal structure. However, knock-down of Unc-5 with repo-Gal4 caused short α/β lobes (arrowhead). c. The percentage of brain hemispheres with short α/β lobes (OK107-Gal4, n = 30; repo-Gal4, n = 28; OK107 > Fra RNAi, n = 25, OK107 > Unc-5 RNAi, n = 20; repo > Fra RNAi, n = 20; repo > Unc-5 RNAi, n = 22, ***p